Accessible luminal interface of bovine rectal organoids generated from cryopreserved biopsy tissues
Fig 5
Electron microscopic characterization of the bovine rectal organoid-derived 2D monolayer.
Representative scanning (A-D) and transmission (E-G) electron microscopy images of 2D monolayer on Day 5 of culture on a cell culture insert. The formation of microvilli on the apical surface of the monolayer was confirmed at low (A) and high (B) power magnifications. A goblet cell was identified on the apical surface of the monolayer at low power magnification (C), where a goblet cell orifice (GO) and microvilli were highlighted at high power magnification (D). (E) A low power magnification image revealed uniformly developed microvilli (MV) on the apical surface and a goblet cell containing multiple mucin granules (MG) in the cytoplasm. N = nucleus. (F&G) A high power magnification images highlight the formation of inter-cellular tight junctions (TJ), desmosome (D) and microvilli (MV) covered with glycocalyx (GLX). Bars, 1 μm.