Response of human peripheral blood monocyte-derived macrophages (PBMM) to demineralized and decellularized bovine bone graft substitutes
Fig 5
Phenotypic analysis of PBMMs polarization after treatment with LPS, IL-4, DMB and DCC substitutes for 8 days.
Phenotypic characterization of macrophages after treatment with bone substitutes was investigated by examining the differential expression of macrophage-related markers like CD14, CD16, CD86, and CD206 based on MFI on gated events. Phenotypic characterization of macrophages after treatment with bone substitutes was investigated by examining the differential expression of macrophage-related markers like CD14, CD16, CD86, and CD206 based on MFI acquired from gated events. One-way ANOVA was used to analyze the data, and the findings were presented as mean + standard error of means. * p<0.05, **< p0.01, *** p<0.001, and **** p<0.0001 is indicated. Error bars represent standard error of means of three independent experiments.