Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis
Fig 6
Peruvioses A and B inhibited pro-inflammatory mediators production by LPS-stimulated RAW 264.7 cells.
Macrophages were cultured with the Peruvioses mixture (0–100 μg/mL) for 30 minutes and then stimulated with LPS (10 μg/mL) for 24 hours. (A) Cell viability was measured with the MTT assay, (B) Nitrite production was measured by the Griess assay, and (C) PGE2, IL-6, TNF-α, and MCP-1 production were measured by ELISA. Results represent at least three independent experiments and are expressed as the mean ± SEM (*) P<0.05, (**) P<0.01, (***) P<0.001, and (****) P<0.0001 vs. control.