Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis
Fig 5
Peruvioses A and B reduce NO and cytokines production probably by inhibition of NF-κB pathway.
Wistar rats instilled with TNBS were treated with the Peruvioses mixture (5 and 10 mg/Kg/day, ip) or vehicle for two weeks, as described in Materials and Methods. (A) Cytokines (IL-1β, IL-6, IL-10, TNF-α, IFN-γ, and IL-4) levels were measured by ELISA. (B) NO production was quantified employing the Griess reaction. (C) Expression of iNOS (cytoplasmic) and NF-κB (nuclear) were evaluated by immunoblotting. Results represent at least two independent experiments and are expressed as the mean ± SEM (n = 7–10 per group). (*) P<0.05, (**) P<0.01, and (****) P<0.0001 vs. control; (+) P<0.05, (+++) P<0.001, and (++++) P<0.0001 vs. TNBS group.