Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis
Fig 4
Treatment with Peruvioses A and B modulates the expression of pro-inflammatory enzymes, cytokines, and NF-κB expression and restores markers of mucus integrity hampered in rats with established TNBS-induced colitis.
Wistar rats instilled with TNBS were treated with the Peruvioses mixture (5 and 10 mg/Kg/day, ip) or vehicle for two weeks, as described in Materials and Methods. (A) Pro-inflammatory enzymes (iNOS, COX-2), (B) Cytokines (IL-1β, IL-6, IL-10, and IL-17A), (C) Mucus integrity markers (MUC-2, MUC-3, and TFF-3), (D) and the transcription factor NF-κB mRNA expression was quantified by RT-PCR. GAPDH was used as a housekeeping gene for normalization. Results represent at least two independent experiments and are expressed as the mean ± SEM (n = 7–10 per group). (*) P<0.05, (**) P<0.01, (***) P<0.001, and (****) P<0.0001 vs. control; (+) P<0.05, (++) P<0.01, (+++) P<0.001, and (++++) P<0.0001 vs. TNBS group.