Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis
Fig 3
Peruvioses A and B pre-treatment reduce the expression of pro-inflammatory enzymes (iNOS, COX-2) and cytokines (TNF-α, IL-10) in acute TNBS-colitis.
Wistar rats were treated with the Peruvioses mixture (5, 10, and 20 mg/Kg/day, ip) or vehicle for 2 days. Afterward, colitis was induced, as described in Materials and Methods. (A) iNOS, COX-2, MUC-2, IL-1β, IL-6, and IL-10 mRNA expression was quantified by RT-PCR. GAPDH was used as a housekeeping gene for normalization. (B) IL-1β, IL-6, IL-10, TNF-α, and IL-4 protein levels were measured by ELISA. Results represent at least two independent experiments and are expressed as the mean ± SEM (n = 9–15 per group). (*) P<0.05, (**) P<0.01, (***) P<0.001, and (****) P<0.0001 vs. control; (+) P<0.05, (++) P<0.01, (+++) P<0.001, and (++++) P<0.0001 vs. TNBS group.