Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis
Fig 2
Peruvioses A and B from P. peruviana diminished the inflammation induced by TNBS.
(A) Colitis was induced in Wistar rats by instillation of TNBS. Animals were treated with the Peruvioses mixture (5 and 10 mg/Kg/day, ip) or vehicle for 15 days. Afterward, rats were sacrificed, and colitis severity was assessed. (B) Survival and (C) body weight changes were monitored daily. (D) Appearance of colon tissue was evaluated, and representative pictures are shown. (E) Macroscopic damage (damaged area (cm2), ulcer index, and colon weight/length) were scored as detailed in Materials and Methods. (F) Myeloperoxidase (MPO) activity was measured in colon biopsies. (G) Histological changes were examined after (1) hematoxylin and eosin (H&E) and (2) Periodic Acid Schiff (PAS) staining. Slides were examined by a blinded pathologist. Representative pictures are shown, magnification 10X (H&E) and 20X (PAS), respectively. Results represent at least two independent experiments and are expressed as the mean ± SEM (n = 10–24 per group). (*) P<0.05, (**) P<0.01, (***) P<0.001, and (****) P<0.0001 vs. control; (+) P<0.05, (++) P<0.01, and (+++) P<0.001 vs. TNBS group.