Protective effect of sucrose esters from cape gooseberry (Physalis peruviana L.) in TNBS-induced colitis
Fig 1
Pre-treatment with Peruvioses A and B from P. peruviana ameliorates acute TNBS-colitis.
(A) Wistar rats were treated for 2 days with test compounds (5, 10, and 20 mg/Kg/day, ip) or vehicle. Colitis was induced by the instillation of TNBS. Three days after that, animals were sacrificed, and colitis severity was assessed. (B) Animals weight change was monitored daily. (C) Appearance, damaged area (cm2), and colon weight/length ratio were evaluated as detailed in Materials and Methods. Representative pictures of rat colons are shown. (D) Histologic changes were examined by hematoxylin and eosin (H&E) staining and scored by a blinded pathologist. Representative pictures are shown (10X). (E) Myeloperoxidase (MPO) activity was measured in colon biopsies. Results represent at least two independent experiments and are expressed as the mean ± SEM. (n = 11–20 per group). (****) P<0.0001 vs. control; (+) P<0.05 and (++) P<0.01 vs. TNBS group.