High-resolution melt curve analysis: An approach for variant detection in the TPO gene of congenital hypothyroid patients in Bangladesh
Fig 5
Normalized melt curves for specimens targeting the c.2173A>C variant in exon 12.
Discernable changes in normalized melt curves were showing that the specimens with homozygous and heterozygous states were clearly distinguishable from the wild-type alleles, as manifested by the difference in relative fluorescence unit.