TK1 expression influences pathogenicity by cell cycle progression, cellular migration, and cellular survival in HCC 1806 breast cancer cells
Fig 9
Exploring the relationship of TK1 on cellular invasion.
I. Wound healing assay comparing migration between L133 and HCC 1806 cells. a. The migration ability of HCC 1806 and L133 cells was performed over a 24-hour period in biological replicates (n = 4). Gap area was normalized prior to analysis. HCC 1806 samples showed faster gap closure than L133 samples using a simple linear regression to compare slopes (p<0.01). b. Visual representation of HCC 1806 and L133 cell migration at hour 1 and hour 6. II. Relative transcript levels in HCC 1806 and L133 cells were quantified using qRT-PCR for a subset of factors involved in apoptosis. Samples were tested in biological replicates (n = 3) and transcript levels were normalized to GAPDH.. Cells were harvested in exponential growth phase under normal cell-culturing conditions. Data are expressed as the mean fold change ± SEM of L133 cells relative to HCC 1806 cells.. L133 cells showed lower expression for all factors tested (p <0.0001). Asterisks in the figure denote the following significance levels: (**) p < 0.01 and (****) p < 0.0001.