Identification of a biosynthetic gene cluster for a red pigment cristazarin produced by a lichen-forming fungus Cladonia metacorallifera
Fig 2
Demarcation of the cristazarin BGC boundaries by mapped reads of RNA-seq.
(A) Mapped reads of five RNA-seq samples of the C. metacorallifera mycobiont growing on different carbon sources were shown for a genomic locus harboring the PKS22 (crz7; gene ID: Cmt_01711). RNA-seq reads mapped on the C. metacorallifera reference genome were subsampled to 60 million reads for visual comparison of expression levels between samples. Arrows on the x-axis indicate genes (Cmt_01704–Cmt_01717). The numbers on the y-axis are per-base coverage of mapped reads. (B) Synteny of the cristazarin BGCs in Cladonia species: C. metacorallifera (top), C. macilenta (middle), and C. borealis (bottom). Arrows indicate open reading frames (ORFs) found in the BGCs, and homologous genes were represented with different colors. Numbers above the arrows indicate crz1–crz9.