High concordance rate of capillary electrophoresis workflow for microsatellite instability analysis and mismatch repair (MMR) immunostaining in colorectal carcinoma
Fig 2
Sensitivities of the MSI-PCR tests using the screening and high-resolution gel capillary electrophoresis.
Genomic DNA of the MSI (+) HCT116 cell line was mixed with that of normal FFPE tissue (A) and fresh frozen tissue (B) in various ratios for the MSI-PCR analysis. The PCR products were visualized by the QIAxcel screening (left) and high-resolution (right) gel electrophoresis for determination of the respective limit of detection (LOD). * indicates the LOD value for each MS marker.