Inhibition of ERK signaling for treatment of ERRα positive TNBC
Fig 6
Inhibition of ERRα together with U0126 prevents cell migration and invasion in TNBC cells.
(A) MDA-MB-231 cells were seeded in a 6-well plate in complete DMEM media and allowed to attach overnight. The following day cells were placed in reduced (1%) serum media and following 8–10 hrs of pretreatment with 10rμM XCT-790, 100nM tamoxifen, or 10rμM U0126, alone or in combination and wound/scratch was generated. Wound closure was measured 17 hrs post generation. (B) Cell migration/Boyden chamber assay was performed as described in “Materials and Methods”. Representative images of MDA-MB-231 cells stained with 4′,6-diamidino-2-phenylindole (DAPI) following 15 hrs migration assay are shown. (C) Quantification of the Wound Healing assay from (A) was performed and graphed using paired Student’s t-test. * represents P<0.05. ** represents P<0.01. (D) Histogram representing the number of cells migrated relative to untreated control. * represents P<0.05, ** represents P<0.01 and *** represents P<0.001.