Inhibition of ERK signaling for treatment of ERRα positive TNBC
Fig 5
Inhibition of ERRα potentiates apoptotic effects of U0126 in TNBC cells.
(A) MDA-MB-231 cells were serum starved for 24 hrs and treated with 10 μM XCT-790, 100 nM tamoxifen, or 10 μM U0126, alone or in combination for 48 hrs. Cells were imaged under 20x magnification using BZX-800 microscope from Keyence. Scale bar represents 100 μm. (B) MDA-MB-231 cells were seeded in 96-well plate in complete DMEM media, supplemented with 10% FBS and treated with 10 μM XCT-790, 100 nM tamoxifen, or 10 μM U0126, alone or in combination for 6 days and cell viability was measured using Neutral Red cytotoxicity assay as described in “Materials and Methods”. (C) MDA-MB-231 cells were serum starved for 24 hrs and treated with 10 μM XCT-790, 100 nM tamoxifen, or 10 μM U0126, alone or in combination for 24 hrs as indicated. Cells were lysed as described in “Materials and Methods” and immunoblotted for indicated proteins. Arrow indicates cleaved PARP fragment. (D) Quantification of cleaved PARP fragment from “B” normalized to actin. * represents P<0.05, ** represents P<0.01 and *** represents P<0.001.