Inhibition of ERK signaling for treatment of ERRα positive TNBC
Fig 4
Tamoxifen potentiates phosphorylation of ERK on Y204/187 in the absence of ERRα.
(A) sh Control and sh ERRα cells were treated with 100 nM of tamoxifen for 5, 15, 30, or 60 minutes. Cells were lysed as described in “Materials and Methods” and the indicated proteins were detected by immunoblot. (B) Relative levels of p-ERK Y204/187 normalized to actin, ** represents P<0.01. (C) Relative levels of p-ERK Y204/187 and ERRα, normalized to actin from ‘A’. (D) MDA-MB 231 cells were treated with 10 μM XCT-790 and/or 100 nM tamoxifen for 30 minutes followed by lysis with NE-PER Nuclear Cytoplasmic extraction kit as described in ‘Materials and Methods’ and lysates from nuclear and cytoplasmic extractions were immunoblotted with the indicated antibodies.