Inhibition of ERK signaling for treatment of ERRα positive TNBC
Fig 3
XCT-790 and tamoxifen treatments upregulate phosphorylation of p-ERK on Y204/187.
(A) MDA-MB-231 cells were grown in starvation media for 24 hrs and treated with either 10 μM XCT-790 or 100 nM tamoxifen for 5, 15, 30 or 60 minutes. Cells were lysed as described in “Materials and Methods” and indicated proteins were detected by immunoblot. (B) Quantification of p-ERK Y204/187 protein levels normalized to total ERK signal from ‘A’. (C) Quantification of p-RSK1 S380 protein levels normalized to actin signal from ‘A’. (D) MDA-MB-231 cells were grown in starvation media for 24 hrs and treated with 10 μM XCT-790 and 100nM tamoxifen for 5, 15, 30 or 60 minutes. Cells were lysed as described in “Materials and Methods” and indicated proteins were detected by immunoblot. (E) Quantification of p-ERK Y204/187 protein levels normalized to actin signal from ‘D’. (F) Quantification of p-RSK1 S380 protein levels normalized to actin signal from ‘D’. * represents P<0.05, ** represents P<0.01 and *** represents P<0.001.