Short-term response to iron resupply in an iron-limited open ocean diatom reveals rapid decay of iron-responsive transcripts
Fig 5
Time course of change in targeted transcript counts in high-iron, low-iron, and iron-recovery samples over the 6 h and the 22 h periods.
Normalized transcript counts are on the y-axes vs time in hours on the x-axes, with 0 h representing 7 AM. Grey boxes indicate the dark phase. The response within the first hour is shown on the left, and the addition of iron is indicated with a red dashed line. Samples from the long-term experiment are shown on the right. High-iron, low-iron, and recovery samples are shown in black, blue, and orange, as in previous figures, with error bars indicating standard errors (S.E.). Transcripts are grouped: (A) iron starvation-induced proteins (ISIP), (B) proteins involved in photosynthesis, (C) Fructose-bisphosphate aldolases (FBA), (D) proteins involved in the glycosylation pathway, (E) transcription factors (HSF, Sigma70TF), iron reductase (FRE1) and a protein of unknown function (CREGx2).