Rare CIDEC coding variants enriched in age-related macular degeneration patients with small low-luminance deficit cause lipid droplet and fat storage defects
Fig 5
AMD CIDEC variants cause a decrease in the lipid exchange rate between lipid droplets (LDs).
(A) Representative Fluorescence Recovery After Photobleach (FRAP) images of paired LD expressing GFP-tagged CIDEC wild-type (WT) showing progressive neutral lipid (BODIPY 558/568 dye labeling) exchange as determined by fluorescence recovery from the adjacent LD. (B) Quantification of mean optical intensity (MOI) in the bleached (blue circle) and unbleached (red circle) LD in cells expressing CIDEC WT or each of the rare variants. (C) Percentage of MOI recovery on bleached LDs from 0 sec. to 300 seconds. N = 3 (mean ± SD, Student’s t test, *p<0.05).