Onopordum acanthium L. extract attenuates pancreatic β-Cells and cardiac inflammation in streptozocin-induced diabetic rats
Fig 5
Effect of GLB or MEOAL on insulin changes in the islets of Langerhans in different groups.
Anti-insulin Immunohistochemical staining (40 X). https://doi.org/10.6084/m9.figshare.21828864.v1. Pancreatic section from; (A) normal control group showing normal distribution of the insulin that occupies the center of the islet (arrow); (B) Diabetic control group showing marked reduction in the immunohistochemical expression of insulin in β-cells (arrow) compared to NC (P<0.05,F); (C) Diabetic GLB(5mg/kg) treated group showing an increase in insulin content of β-cells compared to DC (P>0.05,F); (D) Diabetic MEOAL (200mg/kg) treated group showing an increase of positive reactivity of insulin in the cytoplasm of β-cells (arrow) compared to DC(P>0.05,F); (E) Diabetic MEOAL (400mg/kg) treated group showing significant increase in the expression of insulin in β-cells (arrow) compared to DC (P<0.05,F). F: The bar chart represents anti-insulin immunopositive staining expressed as area%. The results are expressed as mean± ± SEM (n = 8). P-value was calculated using ANOVA with Post Hoc Tukey test. The same letters mean no significant difference, the different letters (a, b, c) mean a significant difference at P<0.05. Abbreviations: NC; normal control group. DC; diabetic control group. GLB; 5mg/kg glibenclamide treated group .200or 400- MEOAL; 200 or 400mg/kg O. acanthium extract treated groups respectively.