Small-molecule inhibitors of proteasome increase CjCas9 protein stability
Fig 2
The proteasome inhibitor MG132 increases CjCas9 protein level.
48 hours after transfection of the cells (in A HEK293T cells and in B HeLa cells) with the pX551-CMV-CjCas9-HA plasmid, the wells were treated with different doses of the proteasome inhibitor MG132 (1 to 5 μM) for 16 hours. The proteins were analyzed 3 days post transfection by western blot with an anti-HA antibody. β-actin was used as a loading control. Data are means ± SEM (n ≥ 3), *p < 0.05, **p < 0.005, and ***p < 0.0005 (Student’s t tests).