Downregulation of COL12A1 and COL13A1 by a selective EP2 receptor agonist, omidenepag, in human trabecular meshwork cells
Fig 2
Effect of omidenepag (OMD) on extracellular matrix (ECM) gene expression in 2D- and 3D-cultured human trabecular meshwork (HTM) cells.
2D- or 3D-cultured HTM cells were treated with vehicle, 10 nM, 100 nM, or 1 μM OMD for 6 or 24 h, and the gene expression changes in ECMs [(a) FN1, COL1A1, COL1A2, and COL4A2, (b) COL12A1, COL13A1, and COL18A1] were assessed using quantitative real-time PCR. The mRNA expression level in each gene was normalized to that for GAPDH and presented as percentages relative to the vehicle-treated group. Each value represents the mean ± S.E. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the vehicle-treated group by Dunnett’s multiple comparison test.