The NLRP6 protein is very faintly expressed in several normal and cancerous epithelial cells and may be confused with an unrelated protein
Fig 2
The endogenous NLRP6 expression is barely detected in several human and mouse cell types by western-blotting using an antibody from ABGENT.
(A) Western-blot analysis of extracts of HCT116 cells transiently transfected by the FLAG-NLRP6-expressing pCDNA3.1+ vector, and of a series of various non-transfected cell lines. The upper image corresponds to a short exposure and the lower image to a longer exposure. (B) Western-blot analysis of the same extracts as in A and total mouse colon extracts. The migration position of NLRP6 is indicated with a dark arrowhead. The white arrowhead points a non-specific band et 9OkDa. The amount of loaded total proteins is indicated for each lane.