Mechanism of LH release after peripheral administration of kisspeptin in cattle
Fig 4
Representative immuno-electron microscopy at the border of median eminence region of medio-basal hypothalamus in a proestrous cow after intravenous kisspeptin administration.
A) black arrows indicate synaptic terminals kisspeptin fiber projection, and white arrows indicate neurosecretory GnRH granules in axon terminals; B) Higher magnification from Fig A (black box) showing kisspeptin immunoreactive axon fiber projection is identified with gold particles (small black dots); C) Higher magnification from Fig A (dotted white rectangle) showing GnRH immunoreactive axon nerve terminal is identified with GnRH neurosecretory granules (DAB staining) surrounded by kisspeptin immunoreactivity (gold particles).