Mechanism of LH release after peripheral administration of kisspeptin in cattle
Fig 3
Distribution of GnRH and c-FOS immunoreactive cells in the preoptic area and hypothalamus after administration of 3 doses of 15 mg human Kisspeptin-10 intravenously (at 1 hr intervals) during proestrous phase of estrous cycle in cows.
A) GnRH immunoreactive neurons (arrow) in the medial preoptic area (mPOA) nucleus showing cytoplasmic DAB (brown) staining. B) GnRH immunoreactive neurons that co-express c-FOS immunoreactive reaction (Nickel-DAB black color). Note that c-FOS is localized in the nucleus (arrow). C-D) GnRH (brown) and c-FOS (black) immunoreaction cells and nerve fibers in the arcuate nucleus and median eminence.