The lymphocyte-specific protein tyrosine kinase-specific inhibitor A-770041 attenuates lung fibrosis via the suppression of TGF-β production in regulatory T-cells
Fig 5
A-770041 inhibit the production of TGF-β in Tregs in vitro.
Tregs obtained from murine spleen were stimulated by anti-CD3/CD28 antibodies and incubated with different concentrations of A-770041 (0, 100, 500 nM) for 24 h. Concentrations of TGF-β in the supernatant were determined by an ELISA (n = 4). The expression of Tgfb mRNA in incubated cells was examined by qPCR (n = 4).