The lymphocyte-specific protein tyrosine kinase-specific inhibitor A-770041 attenuates lung fibrosis via the suppression of TGF-β production in regulatory T-cells
Fig 1
Nintedanib and A-770041 inhibit the phosphorylation of lymphocyte-specific protein tyrosine kinase (Lck) of murine CD4+ T-cells.
CD4+ T-cells were collected from murine spleens using magnetic activated cell sorting. CD4+ T-cells were stimulated by anti-CD3/CD28 antibodies and incubated with different concentrations of nintedanib (0–300 nM) (A)(B) or A-770041 (0–1000 nM) (C)(D) for 5 minutes. The phosphorylation of Lck was analyzed by the Simple WesTM system (n = 4). *p<0.05 versus groups treated without nintedanib or A-770041; **p<0.01 versus groups treated without nintedanib or A-770041.