On taming the effect of transcript level intra-condition count variation during differential expression analysis: A story of dogs, foxes and wolves
Fig 5
Effects of removing transcripts above specified levels of intra-condition variation on differential expression analysis.
a) Number of differentially expressed transcripts (DETs) identified using non-filtered (NF) and filtered datasets, based on the top 10 percentiles (99th to the 90th), for both case studies. Up and down regulated transcripts are represented by red and blue dots respectively. Gray arrows identify the selected thresholds for which the results of subsequent corresponding differential expression analysis were used for the identification of candidate transcript associated with tameness within each case study. b) Number of transcripts identified as differentially expressed within the non-filtered datasets that were associated the highest levels of intra-condition variation (99th to 90th) within both case studies, wolves and dogs (orange dots), and tame and aggressive foxes (blue dots).