CCT196969 effectively inhibits growth and survival of melanoma brain metastasis cells
Fig 4
CCT196969 induces apoptosis in MBM cell lines.
Annexin V labels apoptotic cells and propidium iodide labels necrotic cells. (A) Representative dot plots of the H1 cell line treated with selected concentrations of CCT196969; untreated, 1, 2 and 4 μM. (B) Percentage of viable cells, early apoptotic and late apoptotic and dead cells in the H1, H2 and H3 cell lines. The experiments were done in triplicates. Abbreviations: Q1: Viable cells, Q2: Early apoptotic cells, Q3: Late apoptotic cells, Q4: Necrotic cells. *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001.