Inducing apoptosis using chemical treatment and acidic pH, and detecting it using the Annexin V flow cytometric assay
Fig 1
An example of a flow cytometry plot showing stages of cell death.
Viable cells do not bind Annexin V or take up PI. Cells in early PCD bind Annexin V but still exclude PI. As the cell membrane becomes more permeable, cells in late PCD bind to Annexin V and PI. Dead or necrotic cells do not bind to Annexin V, but do take up PI.