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A rapid and sensitive assay for quantifying the activity of both aerobic and anaerobic ribonucleotide reductases acting upon any or all substrates

Table 2

Average activities +/- standard error of the mean of assays graphically represented in Fig 8.

In addition to the indicated concentration of the allosteric effectors shown below in column 1, components and concentrations were as follows: Trx (30 μM), TrxR (0.5 μM), NADPH (200 μM), CDP (70 μM), UDP (50 μM), GDP (200 μM), ADP (110 μM), NrdA (0.1 μM dimer), NrdB (0.5 μM dimer).

Table 2

doi: https://doi.org/10.1371/journal.pone.0269572.t002