A rapid and sensitive assay for quantifying the activity of both aerobic and anaerobic ribonucleotide reductases acting upon any or all substrates
Fig 4
Activity of E. coli class Ia ribonucleotide reductase.
Each bar represents average activity +/- standard error of the mean for three replicates. NrdA ATP and dATP conditions had 0.1 μM NrdA and 0.5 μM NrdB and 3000 μM ATP or 0.175 μM dATP, respectively, whereas the NrdB condition had 3000 μM ATP, 0.1 μM NrdB, and 0.5 μM NrdA. All conditions contained CDP (1000 μM), Trx (30 μM), TrxR (0.5 μM), and NADPH (200 μM). All protein concentrations are expressed as concentration of the dimer.