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VCP inhibition induces an unfolded protein response and apoptosis in human acute myeloid leukemia cells

Fig 1

Inhibition of VCP leads to accumulation of ubiquitylated proteins and activates the unfolded protein response (UPR) in human acute myeloid leukemia (AML) cells.

Immunoblot showing poly-ubiquitylated proteins in THP-1 cells (A) and in a primary human AML long-term culture (FFM05) (B) after treatment with the VCP inhibitors CB-5083 or NMS-873 or the proteasome inhibitor MG-132. (C) Immunoblot showing expression of UPR-associated proteins in THP-1 and MV4-11 cells after treatment with 200 or 500 nM CB-5083 overnight.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0266478.g001