Accessible, fast and easy fabrication of hydrophilic-in-hydrophobic microdroplet arrays
Fig 5
A) Brightfield micrograph displaying a microdroplet array with 10 μm spot diameter and 25 μm pitch produced in soft contact mode. (B) Brightfield micrograph displaying a microdroplet array with 10 μm spot diameter and 5 μm pitch produced in hard contact mode with the previously published CR-based method [21]. Scale bars are 50 μm. C) Linear regressions showing the correlation between the photomask feature sizes and the droplet sizes obtained by producing the MDA substrates in soft contact with the nLOF-based CR-free method (dashed line, R2 = 0.997, Y = 1.075*X + 2.092), and in hard contact with the previously published CR-based process (full line, R2 = 0.998, Y = 0.877*X + 2.047). The error bars represent standard deviations of 10 repetitions. Droplets on 4 μm diameter spots are not available.