Bergamottin a CYP3A inhibitor found in grapefruit juice inhibits prostate cancer cell growth by downregulating androgen receptor signaling and promoting G0/G1 cell cycle block and apoptosis
Fig 3
Bergamottin treatment causes accumulation of G0/G1 cells similar to CYP3A5 siRNA treated cells.
Watson Pragmatic cell cycle analysis showing frequency of G0/G1, S, and G2/M population in the vehicle control (DMSO), bergamottin and siRNA treated cells. (A and B) Cells were treated with indicated concentration of bergamottin for indicated time points. The cell cycle analysis was performed as described in methods and the analysis was done using FlowJo software, a representative experiment is shown with Watson pragmatic analysis. The average frequency of each population and SD values (±) were calculated after analysing the replicates (minimum of three) and is indicated next to each treatment conditions. (*)- indicates that the P values was less than 0.05 when compare with the respective controls. (C)- Cells were treated with either non target (NT) pool siRNA or CYP3A5 siRNA pool. The analysis method is same as in A and B.