Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
Fig 8
Chemically induced protein crosslinking is initiated when chlorpyrifos oxon makes a covalent bond with lysine to make diethoxyphospho-lysine.
The activated diethoxyphospho-lysine reacts with a nearby glutamic acid or aspartic acid to make an isopeptide bond with release of diethoxyphosphate. The KE and KD crosslinks are resistant to trypsin.