Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
Fig 7
MS/MS spectrum of a diethoxyphospho-adduct on lysine 163 from tubulin alpha-1A.
A). Blue masses define a y-ion sequence. Red masses define a b-ion sequence. Unlabeled blue and red lines represent fragments minus NH3, H2O or CO. The structures at 220.1 and 237.1 m/z represent characteristic ions for diethoxyphospho-lysine. B) Green masses define the b-ion sequence for K(dep)LSVDYGK, the rearranged form of the original peptide.