Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
Fig 6
MS/MS spectrum of an isopeptide crosslink between K163 of tubulin alpha-1A and E158 of tubulin beta-4B induced by treatment of MAP-rich tubulin Sus scrofa with 100 μM chlorpyrifos oxon.
The parent ion at 566.79 is in charge state +3. Arrows point to 14 crosslink-specific fragment ions. Structures are shown for 8 crosslink-specific fragment ions. Fragment ions from both peptides are present. The Protein Prospector score is 29.8 and the score difference is 6.2. The matched intensity is 47.1%. Unassigned, black peaks could not be fit to any sequence.