Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
Fig 3
Immunopurified isopeptide from MAP-rich tubulin.
Panel A shows that immunopurification on Dynabeads-Protein G-anti-isopeptide 81D1C2 yielded a single peptide eluting at 20.24 min. Panel B is the MS/MS spectrum of the immunopurified, naturally-occurring isopeptide in MAP-rich tubulin. The crosslink is between K368 of Tau and Q112 of NFH. The quadruply-charged parent ion is at 401.96 m/z.