Impact of nanodisc lipid composition on cell-free expression of proton-coupled folate transporter
Fig 2
Schematic for cell-free expression of PCFT in the presence of nanodiscs.
T7 S30 Extract, Circular, and S30 Premix Plus are components of the S30 T7 High-Yield Protein Expression System purchased from Promega. The pEXP5-PCFT plasmid is the PCFT construct shown in Fig 1. Empty LPNs containing POPC, DMPG, or DMPC were added to a cell-free reaction mixture. Soluble PCFT was observed when PCFT was cell-free expressed in the presence of the nanodisc.