Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment—In vivo studies
Fig 1
Relationship between lung tissue concentration of CRAMP and dose of synthetic cathelicidin use for inhalation.
Protein concentration was investigated in homogenates of mouse lungs collected from untreated mice and from animals after a single exposure to synthetic cathelicidin at concentration 4, 6 or 8 times higher than physiological. Each single inhalation included 1 h exposure to PBS and 1 h exposure to appropriate concentration of synthetic cathelicidin. CRAMP concentration was determined using the ELISA method. Linear regression analysis of CRAMP concentration in response to synthetic cathelicidin inhalation was conducted. Studies were conducted in 4 research groups, each of which contained 4 animals. Each sample was analyzed in 4 replications. (control mice N = 4; treated mice per research group N = 4).