GSK3ß inhibitor CHIR 99021 modulates cerebral organoid development through dose-dependent regulation of apoptosis, proliferation, differentiation and migration
Fig 3
GSK3β inhibition by CHIR 99021 affects neuronal differentiation of neuroepithelium in a dose dependent manner-Western Blots.
A. Western Blot bands and B. quantification of neuroepithelium marker E-cadherin (E-Cad), neural progenitor cells (NPCs) marker SOX2, radial glia marker BLBP, intermediate progenitor marker TBR2, and neuronal markers TUJ1 and DCX, relative to DMSO control (n = 5 batches of organoid production for the three groups. E-cadherin: DMSO: 1 ± 0.0891; 1μM: 0.9285 ± 0.2585, p-value = 0.2881; 10μM: 1.819 ± 0.5351, *: p-value = 0.0286; SOX2: DMSO: 1 ± 0.09242; 1μM: 1.472 ± 0.1668, *:p-value = 0.0125; 10μM: 0.5228 ± 0.05321, *:p-value = 0.0405; BLBP: DMSO: 1 ± 0.01525; 1μM: 2.069 ± 0.7181, *:p-value = 0.0286; 10μM: 0.6944 ± 0.1048, p-value = 0.8825; TBR2: DMSO: 1 ± 0.1263; 1μM: 1.141 ± 0.09633, p-value = 0.4000; 10μM: 2.884 ± 0.3168, ***: p-value = 0.0006; TUJ1: DMSO: 1 ± 0.1075; 1μM: 0.9262 ± 0.04413, p-value = 0.5429; 10μM: 0.7731 ± 0.0886, p-value = 0.0823; DCX: DMSO: 1 ± 0.2530; 1μM: 1.864 ± 0.4717, p-value = 0.1449; 10μM: -1.516 ± 0.0886, p-value = 0.0044, unpaired t-tests).