Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp.
Fig 7
Ten-fold dilution S. salmonis DNA ranging from 1 fg to 100 ng were used in the presence of 1000X SYBR Green I. Color changes from golden brown to green indicates a positive reaction. The concentrations of template DNA are indicated, NTC = no template control, represents a negative control. The reactions were performed in triplicate and were repeated three times.