Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp.
Fig 6
Optimization of Mg2+ concentration for LAMP.
The concentrations of Mg2+ ranging from 2 mm-10 mM were tested. Top panel represents positive controls using 2ng/μl S. salmonis gDNA. The bottom panel, No Template Control (NTC) represents negative controls. Color change to green indicates positive reaction, while golden brown indicates negative reaction. The experiments were performed in triplicate and repeated three times.