Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp.
Fig 5
A. Schematic representation of the ITS region, showing the positioning of the LAMP primers used in the present study. Scale bar represents 100bp. FwdOut = Forward Outer Primer; RevOut = Backward Outer Primer; F2+F1c = Forward Inner Primer; B2c+B1 = Backward Inner Primer; LF = Loop Forward Primer; LB = Loop Backward Primer. B. Positioning and orientation of LAMP primers developed in the present study. Representative ITS sequences from Saprolegnia and non-Saprolegnia oomycetes were aligned and the LAMP primers were positioned on the alignment manually. The LAMP primers amplified the ITS1 regions of the target sequences.