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Development and comparison of loop-mediated isothermal amplification with quantitative PCR for the specific detection of Saprolegnia spp.

Fig 4

Standard curve of CoxI gene.

Ten-fold dilutions of S. salmonis DNA ranging from 1 fg to 100 ng were amplified in triplicate and the reactions were repeated three times. The iTaq Universal SYBR® Green Supermix and qCOXF1+R1 primers were used.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0250808.g004