Larazotide acetate induces recovery of ischemia-injured porcine jejunum via repair of tight junctions
Fig 4
Expression of tight junction proteins in LA-treated ischemia-injured porcine jejunum.
A: Representative western blot images from cytoplasmic and membrane fractions from ischemia-injured porcine jejunum. Lanes for panel A are as follows: 1: non-ischemic (unrecovered), 2: ischemic (unrecovered), 3: non-ischemic (recovered), 4: ischemic (recovered), 5: ischemic + 1 μM LA (recovered) B: Densitometry analysis of western blot bands for claudin-4 from cytoplasmic and membrane protein fractions of represented tissues. Claudin-4 expected band size using this monoclonal antibody is ~18 kDa. β-actin loading control band size is expected at ~42 kDa. Statistical significance (**) is between ischemic + LA (1 μM) (recovered) tissues vs untreated ischemic (recovered) tissues. Values are means ± SEM; n = 3 for each group.