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Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201

Fig 3

Cancer-related peptides that do not comply with the initial binding consensus are recognized by T1-116C.

Peptides derived from cancer-related proteins that have an arginine at position 1 (R1), but that did not match the T1-116C binding consensus, were synthesized and tested in T2 assays. An irrelevant peptide derived from influenza A virus (GILGFTFVL) was used as a negative control. HLA-A2 expression was detected using the BB7.2 antibody. One representative experiment of three independent biological replicates is shown.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0249967.g003