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Parameters of biliary hydrodynamic injection during endoscopic retrograde cholangio-pancreatography in pigs for applications in gene delivery

Fig 3

Intrabiliary pressure monitored during hydrodynamic injection elucidates differences among the injection parameters.

Baseline pressure within the biliary system is minimal before injection in all conditions (A-D). Shortly before injection, the balloon is inflated creating a seal which appears to have minimal effect on the measured pressure. Upon initiation of injection (solid black arrow, parameters provided above graphs), pressure increases to a short peak, before equilibration during flow at a slightly lower pressure. Cessation of injection (dashed black arrows) yields a sharp decrease in pressure. The deflation of balloon (solid grey arrows) drops pressure further, suggesting a measure of baseline hydrostatic pressure remains in the system after the injection is completed. Higher pressure was achieved at the highest flow rate (D).

Fig 3

doi: https://doi.org/10.1371/journal.pone.0249931.g003