Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome
Fig 5
Basal body separation after knockdown of TbCK1.2.
Knockdown of TbCK1.2 was induced with tetracycline (1 μg/mL for 24 h), and the distance between mature basal body pairs was measured. Antibody against TbRP2 (YL1/2) was used to identify basal bodies. ImageJ was used to measure inter-basal body distances (distance between two YL1/2+ mBBs) in TbCK1.2 RNAi cells cultured in the absence (-Tet) or presence of tetracycline (+Tet). A Mann-Whitney U test was used to determine whether differences in inter-basal body distances in control and TbCK1.2 RNAi cells were statistically significant; p values are noted on the graph. Data presented is an aggregate from six biological replicates (n = 302 (-Tet 1K1N), 183 (+Tet 1K1N), 96 (+Tet 1K2N), 111 (-Tet 2K1N), 61 (+Tet 2K1N), 88 (-Tet 2K2N), 51 (+Tet 2K2N)). Descriptive statistics are provided beneath the corresponding data on each graph.