Casein kinase TbCK1.2 regulates division of kinetoplast DNA, and movement of basal bodies in the African trypanosome
Fig 3
Basal bodies are duplicated after knockdown of TbCK1.2.
Knockdown of TbCK1.2 was induced with tetracycline (1 μg/mL for 24 h) and the effect on basal body duplication was determined using antibodies against TbRP2 (YL1/2, mature basal bodies) or TbSAS6 (detects mature and probasal bodies). DAPI was used to stain DNA. (A) Images represent 1K1N trypanosomes from control cells (-Tet) or 1K2N cells from TbCK1.2-depleted cells (+Tet) with two (2BB) or more (>2BB) basal bodies. Boxed regions are enlarged in the final panel. The average percentage of cell types with the indicated number of mature basal bodies (mBB, YL1/2+) is shown for 1K1N (B) or 1K2N trypanosomes (C) from three independent experiments (n = 108-128/experiment). Blue and red symbols indicate control and RNAi treated samples, respectively. Error bars denote standard deviation. The distribution of mBBs in 1K1N trypanosomes after knockdown of TbCK1.2 was compared to control cells (-Tet) using a χ2 test (1K1N p = 9.1 x 10−26). (A χ2 test could not performed for the 1K2N population because those cells were undetectable (< 1%) in the uninduced population).